837 black & white illustrations, 142 black & white tables
Unsewn / adhesive bound
Table Of Contents
Preface Acknowledgment Author Introduction Section I: Biological Chemistry Elements of Organic Chemistry Introduction Thermodynamic and Chemical Properties of Solutions Thermodynamic State Functions Chemical Properties of Solutions Chemical Equilibrium in Reactions among Solutes Reaction Kinetics Catalysis Solvability in a Polar Solvent Organic Chemistry Building Blocks Hydrocarbons: Types and Structure Functional Groups Polymers Polymerization and Polymer Types Polymeric Materials Structure Polymers for Microfabrication Main Properties Microscopic Models of Macromolecule Solutions Implicit Solvent Models for Solution in Equilibrium Chemical Reactions between Macromolecules: The Perfect Gas of Macromolecules Collisions and Reaction Kinetics References Elements of Biochemistry Introduction Structural Organization of Biochemical Macromolecules General Properties of Macromolecules Organization and Structure of the Cell Viruses Classes of Biological Macromolecules Protein Structure and Chemistry Protein Chemical Structure Protein Stereography: Secondary Structure Protein Stereography: Tertiary Structure Role of Proteins in Biochemistry Immunoglobulin Immunoglobulin G Structure Classification of Human Immunoglobulin Enzymatic Catalysis The Basic Principle of Enzymes Working Kinetics of Enzyme-Catalyzed Reactions Dependency of Enzyme Kinetics on Enzyme Type and Environment Enzyme Inhibition Enzymatic Catalysis Thermodynamics Nucleic Acids Deoxyribonucleic Acid Ribonucleic Acid Structure RNA Types and Roles Lipids Free Fatty Acids Triacylglycerols Membrane Lipids Steroids Carbohydrates Monosaccharides Oligosaccharides Glycoproteins References Biochemical Assays and Sequencing Techniques Introduction Assay Procedure and Preparation Cells Lysis Techniques Nucleic Acid Extraction from Cell Lysates Protein Extraction from Cell Lysates Protein Hydrolysis DNA Amplification by Polymerase Chain Reaction PCR Efficiency PCR Alternative Procedures Enzymatic Assays Detection Methods in Enzymatic Assays Chromatography Liquid Column Chromatography High-Performance Liquid Chromatography Alternatives to Adsorption Liquid-Phase Chromatography Electrophoresis Electrophoresis Gel Types Protein Electrophoresis Nucleic Acid Electrophoresis Immunoassays Structure and Thermodynamic of Antigen-Antibody Neutralization Kinetic of Antigen-Antibody Neutralization Immunoassay Processes Western Blot or Immunoelectrophoresis Enzyme-Linked Immunosorbent Assay Flow Cytometry Assay Nucleic Acid Sequencing First-Generation Fragment Sequencing Second-Generation Fragment Sequencing Third-Generation Fragment Sequencing Fragmenting and Assembly Methods Protein Sequencing and Structural Assessment Protein Sequencing Protein Structure Assessment References Section II: Lab-on-Chip Technology Planar Technology Introduction Planar Process Flow of a Lab on Chip Front-End Process Flow Back-End Process Flow Production Testing Techniques In-Field Testing Micro- and Nano-Fabrication Fabs Clean Rooms Fabrication Materials: Silicon, Silica on Silicon and Pure Silica Wafers Planar Technology Cost Model Industrial Cost Models Industrial Cost Estimation for Microfluidic-Based Labs on Chip Photolithography Wafer Cleaning Photoresist Deposition Mask Alignment Photoresist Exposure Post-Exposure Processes Photolithography Definition Electron Beam Lithography Etching Wet Etching Techniques Plasma Characteristics and Plasma Generation for Planar Plasma Characteristics and Plasma Generation for Planar Processes Dry Etching Techniques Deposition Chemical Vapor Deposition Physical Vapor Deposition Other Physical Depositio
Eugenio Iannone is CEO and founder of Dianax S.R.L., a start-up company operating in the lab-on-chip field. He is an IEEE Engineering in Medicine and Biology Society and American Chemical Society member, as well as an author of three books and over 100 peer-reviewed journal papers. After receiving the Italian Laurea degree in engineering (classical version) from Sapienza - Universita di Roma, he served as a researcher at Fondazione Ugo Bordoni, joined Pirelli s.p.a., and subsequently worked at Cisco. Since 2009, his research has focused on labs on chip. In 2011, he founded the Dianax Study Group, dedicated to lab-on-chip research.