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About this product
- PublisherHumana Press Inc.
- Date of Publication20/05/2016
- GenreLife Sciences: General
- Series TitleMethods in Molecular Biology
- Series Part/Volume Number1413
- Place of PublicationTotowa, NJ
- Country of PublicationUnited States
- ImprintHumana Press Inc.
- Content Note49 Tables, color; 8 Tables, black and white; 52 Illustrations, color; 27 Illustrations, black and white; XIII, 424 p. 79 illus., 52 illus. in color.
- Weight1015 g
- Width178 mm
- Height254 mm
- Edited byPaul Chang
- Edition Statement1st ed. 2016
- Table Of ContentsPart I Methods Focused on Cytology 1: Using Fluorescence Microscopy to Study Mitosis|Sai K. Balchand, Barbara J. Mann, and Patricia Wadsworth2: Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome SegregationBin He and Daniela Cimini Part II Methods Focused on Microtubules and the Mitotic Spindle 3: Purification and Fluorescent Labeling of Tubulin from Xenopus Laevis Egg ExtractsAaron C. Groen and Timothy J. Mitchison4: Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In VitroMarija Zanic5: Imaging and Quantifying the Dynamics of γ-Tubulin at Microtubule Minus Ends in Mitotic SpindlesNicolas Lecland and Jens Luders6: Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF MicroscopyMatthew King and Sabine Petry7: Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions in Synthetic Cell-Like Compartments with Tunable SizeMatthew C. GoodPart III Methods Focused on Kinetochores and the Kinetochore-Microtubule Interface8: In Vitro Kinetochore AssemblyMatthew D. D. Miell and Aaron F. Straight9: Biochemical and Structural Analysis of Kinetochore Histone-Fold ComplexesTatsuya Nishino and Tatsuo Fukagawa10: Measuring Kinetochore-Microtubule Attachment Stability in Cultured CellsKeith F. DeLuca, Jacob A. Herman, and Jennifer G. DeLuca11: Studying Kinetochores In Vivo Using FLIM-FRETTae Yeon Yoo and Daniel J. NeedlemanSection IV Methods Focused on the Spindle Pole12: Purification of Fluorescently Labeled Saccharomyces Cerevisiae Spindle Pole BodiesKimberly K. Fong, Beth Graczyk, and Trisha N. Davis13: A Cell-Free System for Real-Time Analyses of Centriole Disengagement and Centriole to Centrosome ConversionRajesh Kumar Soni and Meng-Fu Bryan Tsou14: Assays to Study Mitotic Centrosome and Spindle Pole Assembly and RegulationVladimir Joukov, Johannes C. Walter, and Arcangela De NicoloPart V Methods Focused on the Cellular Functions of Microtubule Motor Proteins15: Analyzing Spindle Positioning Dynamics in Cultured CellsTomomi Kiyomitsu16: Quantification of Mitotic Chromosome AlignmentCindy Fonseca and Jason Stumpff17: Imaging Mitosis in the Moss Physcomitrella PatensMoe Yamada, Tomohiro Miki, and Gohta Goshima18: Small Molecule Approach to Study the Function of Mitotic KinesinsNaowras Al-Obaidi, Johanna Kastl, and Thomas U. MayerPart VI Novel Approaches to Study Spindle Function and Regulation19: Identification and Characterization of Mitotic Spindle-Localized TranscriptsAmy B. Emerman, Ashwini Jambhekar, and Michael D. Blower20: Probing Mitosis by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible DimerizationEdward R. Ballister and Michael A. Lampson21: Studying Kinetochore KinasesAdrian T. Saurin and Geert J.P.L. Kops22: Engineering and Functional Analysis of Mitotic Kinases through Chemical GeneticsMathew J.K. Jones and Prasad V. JallepalliPart VII The Mitotic Spindle and Cancer23: Using Cell Culture Models of Centrosome Amplification to Study Centrosome Clustering in CancerMijung Kwon24: Generation and Purification of Tetraploid CellsElizabeth M. Shenk and Neil J. Ganem25: Anti-Microtubule DrugsStefan Florian and Timothy J. Mitchison
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