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31 black & white illustrations, 52 colour illustrations, 42 black & white tables, biography
Alberto Sanchez-Diaz, Pilar Perez
Table Of Contents
1. Time-lapse Fluorescence Microscopy Of Budding Yeast Cells Arun Kumar and Manuel Mendoza 2. Real-time Visualization and Quantification of Contractile Ring Proteins in Single Living Cells Reshma Davidson, Yajun Liu, Kenneth S. Gerien, and Jian-Qiu Wu 3. Fluorescence Recovery After Photo-bleaching (FRAP) and Fluorescence Loss in Photo-bleaching (FLIP) Experiments to Study Protein Dynamics during Budding Yeast Cell Division Alessio Bolognesi, Andrzjei Sliwa-Gonzalez, Rupali Prasad, and Yves Barral 4. High-Speed Super Resolution Imaging of Live Fission Yeast Cells Caroline Laplante, Fang Huang, Joerg Bewersdorf, and Thomas D. Pollard 5. Monitoring Chitin Deposition during Septum Assembly in Budding Yeast Arcones, Irene and Roncero Cesar 6. Imaging Septum Formation by Fluorescence Microscopy J.C. Ribas and J.C.G. Cortes 7. Visualization of Cytokinesis Events in Budding Yeast by Transmission Electron Microscopy Franz Meitinger and Gislene Pereira 8. Visualization of Fission Yeast Cells By Transmission Electron Microscopy Matthias Sipiczki 9. Characterization of Septin Ultrastructure in Budding Yeast Using Electron Tomography Aurelie Bertin and Eva Nogales 10. Isolation of Cytokinetic Actomyosin Rings from Saccharomyces cerevisiae and Schizosaccharomyces pombe Junqi Huang, Mithilesh Mishra, Saravanan Palani, Ting Gang Chew, Mohan K. Balasubramanian 11. Measurements of Myosin-II motor Activity during Cytokinesis In Fission Yeast Qing Tang, Luther W. Pollard, and Matthew Lord 12. In Vitro Biochemical Characterization of Cytokinesis Actin Binding Proteins Dennis Zimmermann, Alisha N. Morganthaler, David R. Kovar, and Cristian Suarez 13. Characterization of Ctokinetic F-BARs and other Membrane Binding Proteins Nathan A. McDonald and Kathleen L. Gould 14. Analysis of Three-Dimensional Structures of Exocyst Components Johannes Lesigang and Gang Dong 15. Analysis of Rho-GTPase Activity during Budding-yeast Cytokinesis Masayuki Onishi and John R. Pringle 16. Detection of Phosphorylation Status of Cytokinetic Components Franz Meitinger, Saravanan Palani, and Gislene Pereira 17. Studying Protein-Protein Interactions in Budding Yeast Using Co-Immunoprecipitation Magdalena Foltman and Alberto Sanchez-Diaz 18. Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening Of Suppressor Genes Asli Devrekanli and Masato T. Kanemaki 19. Synchronization of the Budding Yeast Saccharomyces cerevisiae Magdalena Foltman, Iago Molist, and Alberto Sanchez-Diaz 20. Fission Yeast Cell Cycle Synchronization Methods Marta Tormos-Perez, Livia Perez-Hidalgo, and Sergio Moreno 21. A Review of Fluorescent Proteins for Use in Yeast Maja Bialecka-Fornal, Tatyana Makushok, and Susanne M. Rafelski 22. Visualisation and Image Analysis of Yeast Cells Steve Bagley 23. Toolbox for Protein Structure Prediction Daniel Barry Roche and Liam James McGuffin 24. From Structure to Function. A Comprehensive Compendium of Tools to Unveil Protein Domains and Understand their Role in Cytokinesis Sergio Rincon and Anne Paoletti