Picture 1 of 1
Picture 1 of 1
2-D Proteome Analysis Protocols by Andrew J Link: New
US $188.13
Approximately£148.31
Condition:
Postage:
Located in: Sparks, Nevada, United States
Delivery:
Estimated between Wed, 26 Jun and Sat, 29 Jun to 43230
Returns:
Payments:
Shop with confidence
Seller assumes all responsibility for this listing.
eBay item number:282828947370
Item specifics
- Condition
- Book Title
- 2-D Proteome Analysis Protocols
- Publication Date
- 1998-09-24
- Pages
- 601
- ISBN
- 9780896035249
- Subject Area
- Science
- Publication Name
- 2D Proteome Analysis Protocols
- Publisher
- Humana Press
- Item Length
- 9.3 in
- Subject
- Life Sciences / Molecular Biology, Life Sciences / Biochemistry, Chemistry / Electrochemistry
- Publication Year
- 1998
- Series
- Methods in Molecular Biology Ser.
- Type
- Textbook
- Format
- Hardcover
- Language
- English
- Item Height
- 0.5 in
- Item Weight
- 38.1 Oz
- Item Width
- 6.1 in
- Number of Pages
- Xvii, 601 Pages
About this product
Product Information
With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics--the study of the expressed part of the genome--has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectromet- based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry--especially in the fields of molecular biology, biochemistry, microbiology, and cell biology--should find this book eminently useful. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.
Product Identifiers
Publisher
Humana Press
ISBN-10
0896035247
ISBN-13
9780896035249
eBay Product ID (ePID)
569779
Product Key Features
Number of Pages
Xvii, 601 Pages
Language
English
Publication Name
2D Proteome Analysis Protocols
Publication Year
1998
Subject
Life Sciences / Molecular Biology, Life Sciences / Biochemistry, Chemistry / Electrochemistry
Type
Textbook
Subject Area
Science
Series
Methods in Molecular Biology Ser.
Format
Hardcover
Dimensions
Item Height
0.5 in
Item Weight
38.1 Oz
Item Length
9.3 in
Item Width
6.1 in
Additional Product Features
Intended Audience
Scholarly & Professional
LCCN
98-038677
Dewey Edition
21
Reviews
"Our readers will find in this book with its 55 chapters, written by 78 highly well-trained specialists, all they need to come to a good result, going out from the theory of the subject to the precise description of the protocols, the instrumentation, to additive notes, the problems and the eventual difficulties. In one word, an excellent and practical book."-Cellular and Molecular Biology "...There are 55 chapters covering everything you really need to know to get you to speed in the analysis of the proteome. I really liked the layout of each chapter with a pithy introduction followed by materials and methods sections...an absolute must for the proteomic laboratory."-Microbiology Today ". . .the book covers practical aspects , is well documented, and clearly written . . . recommended for researchers in the field. This collection of protocols is an invaluable starting point for people embarking on attempts to develop new proteomes."-FEBS Letters "The wealth of techniques and helpful hints presented in this book should be useful to both neophytes and advanced researchers engaged in the area of high resolution 2-D protein electrophoresis."-Analytical Biochemistry, "Our readers will find in this book with its 55 chapters, written by 78 highly well-trained specialists, all they need to come to a good result, going out from the theory of the subject to the precise description of the protocols, the instrumentation, to additive notes, the problems and the eventual difficulties. In one word, an excellent and practical book."-Cellular and Molecular Biology"...There are 55 chapters covering everything you really need to know to get you to speed in the analysis of the proteome. I really liked the layout of each chapter with a pithy introduction followed by materials and methods sections...an absolute must for the proteomic laboratory."-Microbiology Today". . .the book covers practical aspects , is well documented, and clearly written . . . recommended for researchers in the field. This collection of protocols is an invaluable starting point for people embarking on attempts to develop new proteomes."-FEBS Letters"The wealth of techniques and helpful hints presented in this book should be useful to both neophytes and advanced researchers engaged in the area of high resolution 2-D protein electrophoresis."-Analytical Biochemistry
Series Volume Number
112
Number of Volumes
1 Vol.
Illustrated
Yes
Dewey Decimal
572/.6
Lc Classification Number
Qd415-436
Table of Content
2-D Protein Gel Electrophoresis.- Solubilization of Proteins in 2-D Electrophoresis.- Preparation of Escherichia coli Samples for 2-D Gel Analysis.- Preparing 2-D Protein Extracts from Yeast.- 2-D Protein Extracts from Drosophila melanogaster.- Preparing 2-D Protein Extracts from Caenorhabditis elegans.- Eukaryotic Cell Labeling and Preparation for 2-D.- Differential Detergent Fractionation of Eukaryotic Cells.- Fractionated Extraction of Total Tissue Proteins from Mouse and Human for 2-D Electrophoresis.- Preparation and Solubilization of Body Fluids for 2-D.- 2-D Electrophoresis of Plant Proteins.- Quantifying Protein in 2-D PAGE Solubilization Buffers.- Measuring the Radioactivity of 2-D Protein Extracts.- Advantages and Disadvantages of Carrier Ampholyte IEF.- 2-D Electrophoresis Using Carrier Ampholytes in the First Dimension (IEF).- Nonequilibrium pH Gel Electrophoresis (NEPHGE).- High-Resolution, 2-D Protein Electrophoresis Using Nondedicated Equipment.- Large-Gel 2-D Electrophoresis.- Advantages of Immobilized pH Gradients.- Casting Immobilized pH Gradients (IPGs).- Analytical IPG-Dalt.- IPG-Dalt of Very Alkaline Proteins.- Running Preparative Carrier Ampholyte and Immobilized pH Gradient IEF Gels for 2-D.- In-Gel Sample Rehydration of Immobilized pH Gradient.- High-Resolution, IPG-Based, Mini Two-Dimensional Gel Electrophoresis.- Horizontal SDS-PAGE for IPG-Dalt.- Casting and Running Vertical Slab-Gel Electrophoresis for 2D-PAGE.- Nonreducing 2-D Polyacrylamide Gel Electrophoresis.- 2-D Diagonal Gel Electrophoresis.- 2-D Phosphopeptide Mapping.- Internal Standards for 2-D.- Autoradiography of 2-D Gels.- Double-Label Analysis.- Silver Staining of 2-D Electrophoresis Gels.- Staining of Preparative 2-D Gels.- Electroblotting of Proteins from 2-D Polyacrylamide Gels.- Detection of Total Proteins on Western Blots of 2-D Polyacrylamide Gels.- Protein Detection Using Reversible Metal Chelate Stains.- Glycoprotein Detection of 2-D Separated Proteins.- Image Acquisition in 2-D Electrophoresis.- Computer Analysis of 2-D Images.- 2-D Databases on the World Wide Web.- Comparing 2-D Electrophoretic Gels Across Internet Databases.- Constructing a 2-D Database for the World Wide Web.- Absolute Quantitation of 2-D Protein Spots.- Generating a Bacterial Genome Inventory.- Immunoaffinity Identification of 2-DE Separated Proteins.- 2-DE Spot Amino Acid Analysis with 9-Fluorenylmethyl Chloroformate.- N-Terminal Amino Acid Sequencing of 2-DE Spots.- Characterizing Proteins from 2-DE Gels by Internal Sequence Analysis of Peptide Fragments.- Obtaining Molecular Weights of Proteins and Their Cleavage Products by Directly Combining Gel Electrophoresis with Mass Spectrometry.- Identification of Proteins by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using Peptide and Fragment Ion Masses.- Sample Preparation Methods for Mass Spectrometric Peptide Mapping Directly from 2-DE Gels.- Protein Identification and Analysis Tools in the ExPASy Server.- Automated Protein Identification Using Microcolumn Liquid Chromatography-Tandem Mass Spectrometry.- Peptide Sequencing of 2-DE Gel-Isolated Proteins by Nanoelectrospray Tandem Mass Spectrometry.
Copyright Date
1999
Item description from the seller
Business seller information
Alibris, Inc.
Rob Lambert
2560 9th St
Ste 215
94710-2565 Berkeley, CA
United States
I certify that all my selling activities will comply with all EU laws and regulations.
Seller assumes all responsibility for this listing.
eBay item number:282828947370
Post to me
Item location:
Sparks, Nevada, United States
Posts to:
Albania, Andorra, Angola, Anguilla, Antigua and Barbuda, Argentina, Armenia, Aruba, Australia, Austria, Azerbaijan Republic, Bahamas, Bahrain, Bangladesh, Belgium, Belize, Benin, Bermuda, Bhutan, Bolivia, Bosnia and Herzegovina, Botswana, Brunei Darussalam, Bulgaria, Burkina Faso, Burundi, Cambodia, Cameroon, Canada, Cape Verde Islands, Cayman Islands, Central African Republic, Chad, Chile, China, Colombia, Costa Rica, Cyprus, Czech Republic, Côte d'Ivoire (Ivory Coast), Democratic Republic of the Congo, Denmark, Djibouti, Dominican Republic, Ecuador, Egypt, El Salvador, Equatorial Guinea, Eritrea, Estonia, Ethiopia, Fiji, Finland, France, Gabon Republic, Gambia, Georgia, Germany, Ghana, Gibraltar, Greece, Greenland, Grenada, Guatemala, Guinea, Guyana, Haiti, Honduras, Hong Kong, Hungary, Iceland, India, Indonesia, Ireland, Israel, Italy, Jamaica, Japan, Jordan, Kazakhstan, Kenya, Kiribati, Kuwait, Kyrgyzstan, Laos, Latvia, Lesotho, Liechtenstein, Lithuania, Luxembourg, Macau, Macedonia, Madagascar, Malawi, Malaysia, Maldives, Mali, Malta, Mauritania, Mauritius, Mexico, Moldova, Monaco, Mongolia, Montenegro, Montserrat, Morocco, Mozambique, Namibia, Nauru, Nepal, Netherlands, New Zealand, Nicaragua, Niger, Norway, Oman, Pakistan, Panama, Papua New Guinea, Paraguay, Peru, Philippines, Poland, Portugal, Qatar, Republic of Croatia, Republic of the Congo, Romania, Rwanda, Saint Kitts-Nevis, Saint Lucia, Saint Vincent and the Grenadines, San Marino, Saudi Arabia, Senegal, Serbia, Seychelles, Sierra Leone, Singapore, Slovakia, Slovenia, Solomon Islands, South Africa, South Korea, Spain, Sri Lanka, Suriname, Swaziland, Sweden, Switzerland, Taiwan, Tajikistan, Tanzania, Thailand, Togo, Tonga, Trinidad and Tobago, Tunisia, Turkey, Turkmenistan, Turks and Caicos Islands, Uganda, United Arab Emirates, United Kingdom, United States, Uruguay, Uzbekistan, Vanuatu, Vatican City State, Vietnam, Wallis and Futuna, Western Samoa, Yemen, Zambia, Zimbabwe
Excludes:
APO/FPO, Afghanistan, Alaska/Hawaii, Algeria, Barbados, Belarus, Brazil, French Guiana, French Polynesia, Guadeloupe, Guinea-Bissau, Iraq, Liberia, Libya, Martinique, New Caledonia, Nigeria, Reunion, Russian Federation, US Protectorates, Ukraine, Venezuela
| Postage and packaging | To | Service | Delivery*See Delivery notes |
|---|---|---|---|
| Free postage | United States | Standard Shipping | Estimated between Wed, 26 Jun and Sat, 29 Jun to 43230 |
| US $5.50 (approx £4.34) | United States | Expedited Shipping | Estimated between Wed, 26 Jun and Mon, 1 Jul to 43230 |
| Dispatch time |
|---|
| Will usually dispatch within 2 working days of receiving cleared payment. |
| Taxes |
|---|
| Seller charges sales tax in |
Sales tax for an item #282828947370
Sales tax for an item #282828947370
Seller collects sales tax/VAT for items dispatched to the following states:
| County | VAT rate |
|---|
Returns policy
| After receiving the item, cancel the purchase within | Refund will be given as | Return postage |
|---|---|---|
| 30 days | Money back | Buyer pays for return postage |
Refer to eBay return policyopens in a new tab or window for more details. You're covered by the eBay Money Back Guaranteeopens in a new tab or window if you receive an item that is not as described in the listing.
The buyer is responsible for return postage costs.
| Return policy details |
|---|
| Returns accepted |
Most purchases from business sellers are protected by the Consumer Contract Regulations 2013 which give you the right to cancel the purchase within 14 days after the day you receive the item. Find out more about your rights as a buyer and exceptions.
Payment details
Payment methods
Registered as a business seller
Seller Feedback (463,161)
e***l (97)- Feedback left by buyer.
Past year
Verified purchase
Super impressed with this seller! They had the best price by far for this book! Both the cost of the book and the shipping fee was far better than average! The book came exactly as described, it was well packaged and quite a bit earlier than expected! The seller was polite and replied quickly to my messages.
I’ll definitely make another purchase soon!
Thank you to the seller! Be safe and keep up the great work!
l***w (85)- Feedback left by buyer.
Past 6 months
Verified purchase
WONDERFUL book! Better than described, in just like new condition [not just good]. Packaged very carefully, shipped very securely. Great seller, would buy from again in a heartbeat! No communication occurred, but I imagine it would have been as good as the rest of their customer service. Very happy with purchase, thank you very much!
t***1 (95)- Feedback left by buyer.
Past 6 months
Verified purchase
Excellent packaging, expedited shipping, great seller communication, and accurate photo representation. I'm very pleased to have received this graphic novel for a fair and competitive price. Brand new and sealed!! I would highly recommend and purchase from this reputable retailer again. Thank you 👍!
Product ratings and reviews
More to explore:
- Business Analysis Non-Fiction Paperback Fiction & Books,
- Non-Fiction Business Analysis Fiction & Non-Fiction Books,
- V.C. Andrews Fiction & Fiction Books,
- Andrew Greeley Fiction & Fiction Books,
- Andrew Vachss Fiction & Fiction Books,
- V.C. Andrews Fiction & Fiction Books in English,
- Andrew Gross Paperback Fiction & Fiction Books,
- I-D Magazines,
- Weekly News New Magazines,
- The New Yorker News Magazines