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PCR Primer Design by Anton Yuryev (English) Paperback Book
US $189.32
Approximately£142.39
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eBay item number:386720694880
Item specifics
- Condition
- ISBN-13
- 9781617377433
- Book Title
- PCR Primer Design
- ISBN
- 9781617377433
About this product
Product Identifiers
Publisher
Humana Press
ISBN-10
1617377430
ISBN-13
9781617377433
eBay Product ID (ePID)
109069752
Product Key Features
Number of Pages
Xiv, 432 Pages
Publication Name
Pcr Primer Design
Language
English
Publication Year
2010
Subject
Life Sciences / Molecular Biology, Life Sciences / Cell Biology, Genetics
Type
Textbook
Subject Area
Science, Medical
Series
Methods in Molecular Biology Ser.
Format
Trade Paperback
Dimensions
Item Weight
24.1 Oz
Item Length
9 in
Item Width
6 in
Additional Product Features
Intended Audience
Scholarly & Professional
Dewey Edition
22
Series Volume Number
402
Number of Volumes
1 vol.
Illustrated
Yes
Dewey Decimal
572.86
Table Of Content
Basic Principles and Software for PCR Primer Design.- Physical Principles and Visual-OMP Software for Optimal PCR Design.- OLIGO 7 Primer Analysis Software.- Selection for 3?-End Triplets for Polymerase Chain Reaction Primers.- The Reference Point Method in Primer Design.- PCR Primer Design Using Statistical Modeling.- Developing a Statistical Model for Primer Design.- Genome-Scale PCR Primer Design.- GST-PRIME.- Genome-Scale Probe and Primer Design with PRIMEGENS.- Repeat Masking for PCR Primer Design.- SNPbox.- Fast Masking of Repeated Primer Binding Sites in Eukaryotic Genomes.- Multiplex PCR Primer Design.- Degenerate Primer Design.- An Iterative Method for Selecting Degenerate Multiplex PCR Primers.- Primer Design for Multiplexed Genotyping.- MultiPLX.- MultiPrimer.- Allele-specific PCR.- Modified Oligonucleotides as Tools for Allele-Specific Amplification.- AlleleID.- Long PCR Primer Design.- Designing Primers for Whole Genome PCR Scanning Using the Software Package GenoFrag.- DNA Methylation Mapping.- Designing PCR Primer for DNA Methylation Mapping.- BiSearch.- Graphical Design of Primers with PerlPrimer.
Synopsis
In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes. New high-throughput methods have been developed for genome sequencing and studying the cell at different systematic levels such as transcriptome, proteome, metabolome and other -omes. At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). PCR Primer Design focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, this volume is an exceptional reference for all those involved in studying the genome. In PCR Primer Design, authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome-scale experiments and for small-scale individual PCR amplifications. This volume will be useful for organizations performing whole genome studies, companies designing instruments that utilize PCR, and individual scientists - geneticists, molecular biologists, molecular geneticists, and more - who routinely use PCR in their research., At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). This book focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.
LC Classification Number
QH573-671
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