Techniques in Light Microscopy by Steven E. Ruzin (2024, Hardcover)

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Title: Techniques in Light Microscopy.

About this product

Product Identifiers

PublisherOxford University Press, Incorporated

ISBN-100198885830

ISBN-139780198885832

eBay Product ID (ePID)27071926807

Product Key Features

Number of Pages256 Pages

LanguageEnglish

Publication NameTechniques in Light Microscopy

Publication Year2024

SubjectLife Sciences / Biology, Physics / General

TypeTextbook

AuthorSteven E. Ruzin

Subject AreaScience

FormatHardcover

Dimensions

Item Height0.7 in

Item Weight23.6 Oz

Item Length10 in

Item Width7 in

Additional Product Features

Intended AudienceScholarly & Professional

ReviewsVery well written, clearly describing both traditional, widely-used microscopes and more recent, cutting-edge technologies.

Dewey Edition23

IllustratedYes

Dewey Decimal502.82

Table Of ContentPart I1. Light Microscope Optics2. Lens Aberrations3. The Compound MicroscopePart II4. Transmitted Light Techniques5. Polarized Light Microscopy6. Phase Contrast Microscopy7. Differential Interference Contrast MicroscopyPart III8. Epifluorescence Microscopy9. Selective/Single Plane Imaging Microscopy10. Confocal Microscopy11. Two-Photon Microscopy12. Restoration Microscopy13. Super-Resolution Microscopy14. Resolution15. A Brief History of the Light MicroscopeAppendix I: Digital imaging and samplingAppendix II: Synopsis of transmitted light microscopy techniquesAppendix III: Synopsis of epifluorescence microscopy techniques

SynopsisThe book describes in a simple and readable format the way microscopes magnify images, and all of the techniques that are available today. It covers all the existing epifluorescence techniques including super-resolution and provides historical context on inventions pertaining to the development of the light microscope., This textbook provides an accessible and pedagogical explanation of the way microscopes magnify images and covers all techniques to date in transmitted and fluorescent light microscopy.The first section covers basic optics as it relates to microscopy. The second section describes all the major optical techniques of transmitted light microscopy, starting with brightfield, through darkfield, polarized light, phase contrast, differential interference contrast, and Hoffman modulation contrast microscopy. The final third of the book covers all the techniques of fluorescence microscopy. It begins with a simple explanation of fluorescence and is followed by Widefield epifluorescence, confocal, and 2-photon microscopy. This is followed by computational imaging including restoration ('deconvolution') microscopy, and all the present super-resolution techniques. The book concludes by discussing attainable resolution using transmitted light microscopy, including a thorough discussion of the Rayleigh, Sparrow, and other criteria of resolution, ending with a short discussion of the common pitfalls that result in decreased microscope resolution. The final chapter in the book is a short history of the microscope, beginning with the ancients, then discussing three seminal natural philosophers: Leeuwenhoek, Swammerdam, and Hooke. The remaining sections of the history chapter cover mechanical and optical advancements in the history of transmitted light microscopy. Finally, it includes a short history of fluorescence microscopy starting with Köhler and Rohr's first use of fluorescence microscopy in 1905, and ending with a description of the Sarastro Phoibos 1000, the first commercial confocal microscope.

LC Classification NumberQH207.R8 2024