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Baculovirus: Methods and Protocols by Manon M.J. Cox Hardcover Book
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Item specifics
- Condition
- ISBN-13
- 9781071639603
- Type
- NA
- Publication Name
- NA
- ISBN
- 9781071639603
About this product
Product Identifiers
Publisher
Springer
ISBN-10
1071639609
ISBN-13
9781071639603
eBay Product ID (ePID)
3066540506
Product Key Features
Book Title
Baculovirus : Methods and Protocols
Number of Pages
Xv, 343 Pages
Language
English
Topic
Life Sciences / Microbiology, Life Sciences / Virology, Life Sciences / Zoology / Invertebrates
Publication Year
2024
Illustrator
Yes
Genre
Science
Book Series
Methods in Molecular Biology Ser.
Format
Hardcover
Dimensions
Item Length
10 in
Item Width
7 in
Additional Product Features
Dewey Edition
23
Series Volume Number
2829
Number of Volumes
1 vol.
Dewey Decimal
579.25
Table Of Content
Expansion and Storage of Insect Cells and Virus Stocks.- Construction of Recombinant Baculoviruses.- A Time and Cost-Effective Pipeline for Expression Screening and Protein Production in Insect Cells Based on the HR-Bac Toolbox to Generate Recombinant Baculoviruses.- Optimized Workflow from Gene to Product.- TOPO Cloning for Efficient Plasmid Construction.- Adaptive Laboratory Evolution to Improve Recombinant Protein Production Using Insect Cells.- RNAi-Mediated Silencing in the Insect Cell-Baculovirus Expression System.- Scarless Baculovirus Genome Editing Using Lambda Red Recombineering in E. coli.- Probing Baculovirus Vector Gene Essentiality for Foreign Gene Expression Using a CRISPR-Cas9 System.- Generation of Complex Protein Structures by Co-Infection with High Quality Recombinant Baculovirus.- Production of Secreted Antibody in Baculovirus Expression Vector System.- Production of Recombinant Viral Antigens Using the Baculovirus-Insect Cell Expression System.- Protein Production at the 100L Scale.- Recombinant AAV Production.- Recombinant AAV Purification.- Extraction of Modified Adeno-Associated Virus-Like Particles Displaying Peptides on Their Surface.- One-Step Purification Strategy for Cowpea Chlorotic Mottle Virus-Like Particles Produced by the IC-BEVS.- Purification Process for a Secreted Protein Produced in Insect Cells.- Functional Baculovirus Particle Quantification via Plaque Assay.- TCID50 Assay: A Simple Method to Determine Baculovirus Titer.- Rapid Two-Day Baculovirus Titering Using Flow Cytometry.- A Microsphere Immunoassay for the Quantitative Detection of Antigens in Cell Culture Supernatant.- Non-Viral Platform for Expression of Recombinant Protein in Insect Cells.- Assembly of Baculovirus Vectors for Multiplexed Prime Editing.- Recombinant Protein Production in Suspension Mammalian Cells Using the BacMam Baculovirus Expression System.
Synopsis
This detailed volume provides up-to-date guidance on techniques used to work with baculoviruses and insect cells. The book provides basic methods to create recombinant baculoviruses, to improve productivity, to produce a variety of products, to purify products, to quantify baculovirus stocks or to quantify product produced, and it concludes with alternative uses of either baculovirus or insect cells as tools. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Baculovirus: Methods and Protocols serves as an ideal guide for researchers looking to overcome some of the limitations associated with the early baculoviral vectors and cell lines.
LC Classification Number
QR355-502
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- 6***i (859)- Feedback left by buyer.Past monthVerified purchaseThe book arrived faster than advertised and came in flawless condition since it was so well packed. The price was great and so was the seller's customer service when I contacted them with a question. I would not hesitate to buy from this seller again!
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Seller Feedback (1,051,495)
- 6***i (859)- Feedback left by buyer.Past monthVerified purchaseThe book arrived faster than advertised and came in flawless condition since it was so well packed. The price was great and so was the seller's customer service when I contacted them with a question. I would not hesitate to buy from this seller again!
- y***0 (347)- Feedback left by buyer.Past 6 monthsVerified purchaseA+++++++seller they gave me a great price and shipped it out immediately and communicated friendly. Shipping was fast and when it arrived it was packaged thoughtfully. The book is brand new and exactly as described. I have come to exect high quality books from them as they are always brand new. I definitely recommend this seller to anyone who wants beautiful books at a great price. Thank you for a great transaction.
- l***r (987)- Feedback left by buyer.Past 6 monthsVerified purchaseA Plus Seller: Transaction Good. Tested Good. Packaged Good. Better than described. Shipping reasonable time. Value contents of book: A Plus. True Life story. From one Autistic person to an other. Right on the money. Jesus Does Talk to People today. Are you listening with a Facilitator and the Spirit guiding, leading, asking Questions? I would not have believed it, until I read it. I have seen it in action. Life Change! Extremely Valuable.