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Plant Chromatin Dynamics: Methods and Protocols by Marian Bemer (English) Hardco
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eBay item number:395167140727
Item specifics
- Condition
- ISBN-13
- 9781493973170
- Type
- NA
- Publication Name
- NA
- ISBN
- 9781493973170
- Publication Year
- 2017
- Format
- Hardcover
- Language
- English
- Book Title
- Plant Chromatin Dynamics : Methods and Protocols
- Item Length
- 10in
- Publisher
- Springer New York
- Genre
- Science
- Topic
- Life Sciences / Botany
- Item Width
- 7in
- Item Weight
- 493 Oz
- Number of Pages
- Xiv, 659 Pages
About this product
Product Information
This volume provides a comprehensive collection of protocols that can be used to study plant chromatin structure and composition. Chapters divided into three sections detail the profiling of chromatin features in relation to epigenetic regulation, investigate the interaction between chromatin modifications and gene regulation, and explore the 3D spatial organization of the chromatin inside the nucleus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Chromatin Dynamics: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Product Identifiers
Publisher
Springer New York
ISBN-10
1493973177
ISBN-13
9781493973170
eBay Product ID (ePID)
240569324
Product Key Features
Book Title
Plant Chromatin Dynamics : Methods and Protocols
Format
Hardcover
Language
English
Topic
Life Sciences / Botany
Publication Year
2017
Genre
Science
Number of Pages
Xiv, 659 Pages
Dimensions
Item Length
10in
Item Width
7in
Item Weight
493 Oz
Additional Product Features
Series Volume Number
1675
Number of Volumes
1 Vol.
Lc Classification Number
Qk
Table of Content
Section Overview: Profiling Developmentally and Environmentally Controlled Chromatin Reprogramming.- Profiling DNA Methylation using Bisulfite Sequencing (BS-Seq).- Bisulfite Sequencing using Small DNA Amounts.- Identification of Differentially Methylated Regions in the Genome of Arabidopsis thaliana.- A Rapid and Efficient ChIP Protocol to Profile Chromatin Binding Proteins and Epigenetic Modifications in Arabidopsis.- Sequential ChIP Protocol for Profiling Bivalent Epigenetic Modifications (ReChIP).- A Method to Identify Nucleolus-Associated Chromatin Domains (NADs).- Cell Type-Specific Profiling of Chromatin Modifications and Associated Proteins.- Mapping of Histone Modifications in Plants by Tandem Mass Spectrometry.- Histone H1 Purification and Post-Translational Modification Profiling by High-Resolution Mass Spectrometry.- Profiling Nucleosome Occupancy by MNase-seq: Experimental Protocol and Computational Analysis.- Identification of Open Chromatin Regions in Plant Genomes usingATAC-Seq.- Section Overview: Unravelling the Complex Epigenetic Mechanisms that Regulate Gene Activity.- Technical Review: A Hitchhiker's Guide to Chromosome Conformation Capture.- 3C in Maize and Arabidopsis.- Profiling Histone Modifications in Synchronised Floral Tissues for Quantitative Resolution of Chromatin and Transcriptome Dynamics.- De Novo Identification of sRNA Loci and Non-Coding RNAs by High-Throughput Sequencing.- Identification of in planta Protein-Protein Interactions using IP-MS.- RNA Immunoprecipitation Protocol to Identify Protein-RNA Interactions in Arabidopsis thaliana.- In vitro Assays to Measure Histone Methyltransferase Activity using Different Chromatin Substrates.- Identification of Parent-of-Origin-Dependent QTLs using Bulk-Segregant Sequencing (Bulk-Seq).- QTLepi mapping in Arabidopsis thaliana.- Section Overview: a Compendium of Methods to Analyze the Spatial Organization of Plant Chromatin.- Localisation of Chromatin Marks in Arabidopsis EarlyEmbryos.- Cell-type Specific Chromatin Analysis in Whole-mount Plant Tissues by Immunostaining.- Measuring Dynamics of Histone Proteins by Photobleaching in Arabidopsis Roots.- Fluorescence In Situ Hybridization (FISH) and Immunolabeling on 3D Preserved Nuclei.- High-Affinity LNA/DNA Mixmer Probes for Detection of Chromosome-Specific Polymorphisms of 5S rDNA Repeats in Arabidopsis thaliana.- A Method for Testing Random Spatial Model on Nuclear Object Distributions.- Technical Review: Cytogenetic Tools for Studying Mitotic Chromosomes.- Technical Review: Microscopy and Image Processing Tools to Analyse Plant Chromatin - practical Considerations.- Automated 3D Gene Position Analysis using a Customized Imaris Plugin: XTFISHInsideNucleus.- Quantitative 3D Analysis of Nuclear Morphology and Heterochromatin Organization from Whole Mount Plant Tissue using NucleusJ.- Transmission Electron Microscopy Imaging to Analyse Chromatin Density Distribution at the Nanoscale Level.
Copyright Date
2018
Series
Methods in Molecular Biology Ser.
Illustrated
Yes
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