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Herpes Simplex Virus: Methods and Protocols by Russell J. Diefenbach (English) H
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- Buy it nowHerpes Simplex Virus: Methods and Protocols by Russell J. Diefenbach (English) H
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Located in: Fairfield, Ohio, United States
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eBay item number:396215311722
Item specifics
- Condition
- ISBN-13
- 9781493998135
- Book Title
- Herpes Simplex Virus
- ISBN
- 9781493998135
About this product
Product Identifiers
Publisher
Springer New York
ISBN-10
1493998137
ISBN-13
9781493998135
eBay Product ID (ePID)
10038416574
Product Key Features
Number of Pages
Xiv, 457 Pages
Publication Name
Herpes Simplex Virus : Methods and Protocols
Language
English
Publication Year
2019
Subject
Immunology, Diseases
Type
Textbook
Subject Area
Medical
Series
Methods in Molecular Biology Ser.
Format
Hardcover
Dimensions
Item Weight
38.8 Oz
Item Length
10 in
Item Width
7 in
Additional Product Features
Edition Number
2
Dewey Edition
23
Reviews
"The book is intended to help researchers in the field of herpes simplex virus research in understanding complex interactions between herpes virus and host. In my opinion, in addition to the researchers, the book will be quite useful for students and virologists who wish to further their knowledge in this field. The book does meet the needs of the intended audience by providing comprehensive, cutting-edge information on HSV methods and protocols." (Omer Iqbal, Doody's Book Reviews, October 2, 2020)
Series Volume Number
2060
Number of Volumes
1 vol.
Illustrated
Yes
Dewey Decimal
614.5/812
Table Of Content
Tour de Herpes: Cycling through the Life and Biology of HSV-1.- Vaccines for Herpes Simplex: Recent Progress Driven by Viral and Adjuvant Immunology.- Herpes Simplex Virus Growth, Preparation, and Assay.- Engineering HSV-1 Vectors for Gene Therapy.- Preparation of Herpes Simplex Virus Type 1 (HSV-1)-Based Amplicon Vectors.- HSV-1 Amplicon Vectors as Genetic Vaccines.- oHSV Genome Editing by Means of galK Recombineering.- Rescue, Purification, and Characterization of a Recombinant HSV Expressing a Transgenic Protein.- CRISPR/Cas9-Based Genome Editing of HSV.- Latent/Quiescent Herpes Simplex Virus 1 Genome Detection by Fluorescent In Situ Hybridization (FISH).- Oligonucleotide Enrichment of HSV-1 Genomic DNA from Clinical Specimens for use in High-Throughput Sequencing.- HSV Mutant Generation and Dual-Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo.- Phenotypic and Genotypic Testing of HSV-1 and HSV-2Resistance to Antivirals.- Using Primary SCG Neuron Cultures to Study Molecular Determinants of HSV-1 Latency and Reactivation.- Characterization of Extracellular HSV-1 Virions by Proteomics.- Analysis and Sorting of Individual HSV-1 Particles by Flow Virometry.- Isolation/Analysis of Extracellular Microvesicles from HSV-1-Infected Cells.- Conformational Change in Herpes Simplex Virus Entry Glycoproteins Detected by Dot Blot.- BioID Combined with Mass Spectrometry to Study Herpesvirus Protein-Protein Interaction Networks.- Preparation of Herpes Simplex Virus Infected Primary Neurons for Transmission Electron Microscopy.- Transmission Immuno-Electron Microscopy of Herpes Simplex Virus-1 Infected Dorsal Root Ganglia Neurons Sectioned in Growth Plane.- Multi-Fluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication.- Expression, Purification, and Crystallization of HSV-1 Glycoproteins for Structure Determination.- Expression, Purification, and Crystallization of Full-Length HSV-1 gB for Structure Determination.- The Use of Microfluidic Neuronal Devices to Study the Anterograde Axonal Transport of Herpes Simplex Virus-1.- A Model of In Vivo HSV-1 DNA Transport using Murine Retinal Ganglion Cells.- The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines.
Synopsis
This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR/Cas9 engineering, and the use of BioID to identify protein-protein interactions. Chapters cover topics such as the biology, life cycle, and current state of antiviral and vaccine development for HSV-1; protocols on growing viruses in cell culture and manipulating viral DNA; design and application of HSV-1 vectors for cancer- and gene-therapy; and structural analyses, microscopy, proteomics, and testing of antivirals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Herpes Simplex Virus: Methods and Protocols, Second Edition is a valuable resource for immunologists, and molecular and cell biologists. This book will also be useful for researchers who wish to initiate molecular and/or cellular-based approaches to study HSV.
LC Classification Number
RC1-1245
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- w***i (871)- Feedback left by buyer.Past monthVerified purchaseAbsolutely Wonderful Seller!! Terrific Item As Described!!! Great Service and Communication!! Shipped In Waterproof Packaging!! I Received Item In About One Week!! Very Pleased With Seller! I Will Buy From This Seller Again!!
- r***r (2961)- Feedback left by buyer.Past 6 monthsVerified purchaseBook arrived today, 2 days before deadline given by the seller. Well-packaged & in good condition. Price was very competitive, & the book was accurately listed. Great communication. I appreciate the seller getting back to me when I was having trouble tracking the book's delivery progress. Apparently there were 2 different tracking numbers - one in Canada & the other in the U.S., since it was initially shipped from Canada. Thank you very much for your quick response & helpful information.Rethinking Investing: A Very Short Guide to Very Long-Term Investing by Charles (#396183075510)
- e***_ (15)- Feedback left by buyer.Past 6 monthsVerified purchaseSeller handles a massive inventory so I give them grace on the slow processing and shipping. They were very communicative and prompt with their response when I messaged in asking about it. Book was in good condition as described. Small ding on the page edges which wasn't mentioned, but that's fairly common even at your typical book store. Still a good value and I'd buy from them again.
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Seller Feedback (1,054,301)
- w***i (871)- Feedback left by buyer.Past monthVerified purchaseAbsolutely Wonderful Seller!! Terrific Item As Described!!! Great Service and Communication!! Shipped In Waterproof Packaging!! I Received Item In About One Week!! Very Pleased With Seller! I Will Buy From This Seller Again!!
- r***r (2961)- Feedback left by buyer.Past 6 monthsVerified purchaseBook arrived today, 2 days before deadline given by the seller. Well-packaged & in good condition. Price was very competitive, & the book was accurately listed. Great communication. I appreciate the seller getting back to me when I was having trouble tracking the book's delivery progress. Apparently there were 2 different tracking numbers - one in Canada & the other in the U.S., since it was initially shipped from Canada. Thank you very much for your quick response & helpful information.Rethinking Investing: A Very Short Guide to Very Long-Term Investing by Charles (#396183075510)
- e***_ (15)- Feedback left by buyer.Past 6 monthsVerified purchaseSeller handles a massive inventory so I give them grace on the slow processing and shipping. They were very communicative and prompt with their response when I messaged in asking about it. Book was in good condition as described. Small ding on the page edges which wasn't mentioned, but that's fairly common even at your typical book store. Still a good value and I'd buy from them again.