Reviews"If you are planning to set up any functional cell structure facility, small- or large-scale, Basic Cell Structure could be very useful. The book has high practical and scientific value. . .The text is compact and it is easy to find specific information. . .Overall, the book provides a guide for any university of pharmaceutical company laboratory, with or without good laboratory practise (GLP) standards, that aims to obtain consistent cell biological data. I suggest this book cold serve as a handy reference guide and recommend it for managers of cell structure facilities and any person practising cell structure work. . .[T]his book takes on a wider and more conclusive attitude to the cell structure process. Personally, I have placed it in the lab where I am working in , and my colleagues and I have found it to be very handy when working at the bench or planning future studies."--Journal of Cell Science"The second edition contains a new chapter--on microscopy--updated information throughout the text, and an appendix of Internet sites. Students and professionals in the field of biotechnology will find this a comprehensive, clearly organized guide to laboratory procedures. Each chapter outlines basic principles, detailed discussion of the topic, a list of references, and numerous diagrams and photos. The topics include quality control of cell lines, cloning, specific cell types and their requirements, basic cell culture technique, primary culture, and the establishment and maintenance of cell lines."--SciTech Book News, "If you are planning to set up any functional cell structure facility, small- or large-scale, Basic Cell Structure could be very useful. The book has high practical and scientific value. . .The text is compact and it is easy to find specific information. . .Overall, the book provides a guide for any university of pharmaceutical company laboratory, with or without good laboratory practise (GLP) standards, that aims to obtain consistent cell biological data. I suggest this book cold serve as a handy reference guide and recommend it for managers of cell structure facilities and any person practising cell structure work. . .[T]his book takes on a wider and more conclusive attitude to the cell structure process. Personally, I have placed it in the lab where I am working in , and my colleagues and I have found it to be very handy when working at the bench or planning future studies."--Journal of Cell Science "The second edition contains a new chapter--on microscopy--updated information throughout the text, and an appendix of Internet sites. Students and professionals in the field of biotechnology will find this a comprehensive, clearly organized guide to laboratory procedures. Each chapter outlines basic principles, detailed discussion of the topic, a list of references, and numerous diagrams and photos. The topics include quality control of cell lines, cloning, specific cell types and their requirements, basic cell culture technique, primary culture, and the establishment and maintenance of cell lines."--SciTech Book News, "If you are planning to set up any functional cell structure facility, small- or large-scale, Basic Cell Structure could be very useful. The book has high practical and scientific value. . .The text is compact and it is easy to find specific information. . .Overall, the book provides a guide for any university of pharmaceutical company laboratory, with or without good laboratory practise (GLP) standards, that aims to obtain consistent cell biological data. I suggest this book cold serve as a handy reference guide and recommend it for managers of cell structure facilities and any person practising cell structure work. . .[T]his book takes on a wider and more conclusive attitude to the cell structure process. Personally, I have placed it in the lab where I am working in , and my colleagues and I have found it to be very handy when working at the bench or planning future studies."-- Journal of Cell Science "The second edition contains a new chapter--on microscopy--updated information throughout the text, and an appendix of Internet sites. Students and professionals in the field of biotechnology will find this a comprehensive, clearly organized guide to laboratory procedures. Each chapter outlines basic principles, detailed discussion of the topic, a list of references, and numerous diagrams and photos. The topics include quality control of cell lines, cloning, specific cell types and their requirements, basic cell culture technique, primary culture, and the establishment and maintenance of cell lines."-- SciTech Book News, '"I think that this is a very useful book, and I recommend it not only to beginners, but also to more experienced cell culture workers, who will find many new hints and pieces of information, presented in a readily accessible way."'Experimental Physiology, 'Review from previous edition I think that this is a very useful book, andI recommend it not only to beginners, but also to more experienced cell cultureworkers, who will find many new hints and pieces of information, presented in areadily accessible way.'Experimental Physiology, 81, 1047-1049, '"I think that this is a very useful book, and I recommend it not only tobeginners, but also to more experienced cell culture workers, who will find manynew hints and pieces of information, presented in a readily accessible way."'Experimental Physiology
Series Volume Number254
Table Of Content1: Caroline Wigley: The Cell Culture Laboratory2: Peter Roberts: Sterilization3: T Cartwright and G P Shah: Culture Media4: Ian Dobbie and Daniel Zicha: Microscopy of Living Cells5: James McAteer and John M Davis: Basic cell culture and the maintenance of cell lines6: Caroline McDonald: Primary culture and the establishment of cell lines7: J D Sato et al: Specific cell types and their requirements8: John Clarke, Alison Porter, Robin Thorpe, and John M Davis: Cloning9: B J Bolton, P Packer and A Doyle: The quality control of cell lines10: Barbara Clitherow: Good laboratory practice in the cell culture laboratoryAppendix1: J Dow: Online resources for cell biology
Edition DescriptionRevised edition
SynopsisThis book is a guide for the newcomer to all those areas which are currently basic to the performance of animal (including human) cell culture. Experienced workers should also find it useful as a source of reference when entering a new field, or when setting up a new laboratory., The use of animal, including human, cell culture has expanded enormously during the last 25 years, with new applications appearing all the time. The aim of this book is to guide the newcomer progressively through all those areas which nowadays are basic to the performance of cell culture. The book should also prove useful to the experienced worker when entering a new field, setting up a new laboratory, or as a source of reference on basic techniques.Revised and updated chapters cover topics such as equipping a cell culture laboratory, sterilization, culture media, basic culture technique, cell cloning, the isolation of new cell lines, contamination procedures, and good laboratory practice. There is a brand new chapter on the microscopy of living cells, and an appendix of online resources for cell biology., The use of animal, including human, cell culture has expanded enormously during the last twenty years, with the new applications appearing all the time. This book guides the newcomer progressively through all those areas which are basic to the performance of cell culture. It will also prove useful to the experienced worker entering a new field or setting up a new laboratroy, and as a source of reference on basic techniques. The topics covered include setting up and equipping a cell culture laboratory, sterilization of fluids and equipment, culture media, basic culture technique and the maintenance of cell lines, primary culture and the isolation of new cell lines, specific cell types and their requirements, single cell cloning, quality control of cell lines and the prevention, detection, and cure of contamination., The use of animal, including human, cell culture has expanded enormously during the last 25 years, with new applications appearing all the time. The aim of this book is to guide the newcomer progressively through all those areas which nowadays are basic to the performance of cell culture. The book should also prove useful to the experienced worker when entering a new field, setting up a new laboratory, or as a source of reference on basictechniques.Revised and updated chapters cover topics such as equipping a cell culture laboratory, sterilization, culture media, basic culture technique, cell cloning, the isolation of new cell lines,contamination procedures, and good laboratory practice. There is a brand new chapter on the microscopy of living cells, and an appendix of online resources for cell biology.
LC Classification NumberQH585.2.B37 2001