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Use Instructions:Before Treating: Remove all invertebrates - these are extremely sensitive to copper and will not tolerate treatment with a copper-based medication. Turn off UV filters, ozone filters, and remove chemical filtration like MatrixCarbon™ and Purigen. Do not use in conjunction with any other medication. Do not use any products which contain reducing agents (conditioners, ammonia binders, etc.) while using Cupramine.Treatment: If the bottle has a dropper cap, use 20 drops (1 mL) per 40 L (10.5 US gallons) the first day, wait 48 hours, then repeat. On non-dropper caps, each inner ring is 1 mL. In freshwater use half dose. Final copper concentration is 0.5 mg/L (0.25 mg/L in freshwater). Leave at this concentration for 14 days. Do no.