Picture 1 of 1
Stock photo
Picture 1 of 1
Stock photo
Advances in Experimental Medicine and Biology Ser.: Advanced Technologies for Protein Complex Production and Characterization by M. Cristina Vega (2016, Hardcover)
Great Book Prices Store (336429)
96.6% positive Feedback
Price:
US $220.53
Approximately£162.50
+ $19.99 postage
Returns:
14 days return. Buyer pays for return postage. If you use an eBay delivery label, it will be deducted from your refund amount.
Condition:
- Buy it nowAdvanced Technologies for Protein Complex Production and Characterization, Ha...
Oops! Looks like we're having trouble connecting to our server.
Refresh your browser window to try again.
About this product
Product Identifiers
PublisherSpringer International Publishing A&G
ISBN-103319272144
ISBN-139783319272146
eBay Product ID (ePID)219364383
Product Key Features
Number of PagesXiii, 382 Pages
LanguageEnglish
Publication NameAdvanced Technologies for Protein Complex Production and Characterization
Publication Year2016
SubjectLife Sciences / Molecular Biology, Life Sciences / Biochemistry, Life Sciences / Genetics & Genomics, Chemistry / Organic
TypeTextbook
Subject AreaScience
AuthorM. Cristina Vega
SeriesAdvances in Experimental Medicine and Biology Ser.
FormatHardcover
Dimensions
Item Weight312.4 Oz
Item Length10 in
Item Width7 in
Additional Product Features
Intended AudienceScholarly & Professional
Dewey Edition23
Series Volume Number896
Number of Volumes1 vol.
IllustratedYes
Dewey Decimal572.6
Table Of ContentProtein complex production from the drug discovery standpoint.- Choose a suitable expression host: a survey of available protein production platforms.- ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts.- Complex reconstitution and characterization by combining co-expression techniques in Escherichia coli with high-throughput.- Membrane protein production in E. coli for applications in drug discovery.- Cell-free Synthesis of Macromolecular Complexes.- A Bacillus megaterium system for the production of recombinant proteins and protein complexes.- Protein complex production in alternative prokaryotic hosts.- Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts.- Leishmania tarentolae for the Production of Multi-Subunit Complexes.- Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes.- Fundamentals of baculovirus expression and applications.- The MultiBac Baculovirus / Insect Cell Expression Vector System for Producing Complex Protein Biologics.- Fundamentals of expression in mammalian cells.- Assembling multi-subunit complexes using mammalian expression.- Microalgae as solar-powered protein factories.- Strategies and methodologies for the co-expression of multiple proteins in plants.- Transient expression systems in plants--potentialities and constraints.- Complex reconstitution from individual protein modules.- Structural reconstruction of protein-protein complexes involved in intracellular signaling.- The use of small-angle scattering for the characterization of multi subunit complexes.- Application of nuclear magnetic resonance and hybrid methods to structure determination of complex systems.
SynopsisThis book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli , yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists. Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa ( Leishmania terantolae and Dictyostelium discoideum ), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance. This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies.
LC Classification NumberQD431-431.7
