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Methods in Molecular Biology Ser.: Synapse Development : Methods and Protocols by Alexandros Poulopoulos (2016, Hardcover)

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About this product

Product Identifiers

PublisherSpringer New York

ISBN-101493966863

ISBN-139781493966868

eBay Product ID (ePID)245209880

Product Key Features

Number of PagesXi, 372 Pages

Publication NameSynapse Development : Methods and Protocols

LanguageEnglish

SubjectNeurology, Life Sciences / Neuroscience

Publication Year2016

TypeTextbook

AuthorAlexandros Poulopoulos

Subject AreaScience, Medical

SeriesMethods in Molecular Biology Ser.

FormatHardcover

Dimensions

Item Weight304.6 Oz

Item Length10 in

Item Width7 in

Additional Product Features

Intended AudienceTrade

LCCN2016-956874

Dewey Edition23

Series Volume Number1538

Number of Volumes1 vol.

IllustratedYes

Dewey Decimal612.8

Table Of ContentA Cell Culture System to Investigate the Presynaptic Control of Subsynaptic Membrane Differentiation at the Neuromuscular Junction.- Co-Culture Synaptogenic Assay: A New Look at Fluorescence Reporters and Technological Devices.- Synaptogenic Assays Using Neurons Cultured on Micropatterned Substrates.- Monitoring Synapses Via Trans-Synaptic GFP Complementation.- Generation of Spinal Motor Neurons from Human Pluripotent Stem Cells.- Biochemical Purification of Binding Partners of Synaptic Scaffold Proteins.- In Situ Protein Binding Assay Using Fc-Fusion Proteins.- ation Microscopy for the Investigation of Synaptic Structure and Function.- 3D d STORM Imaging of Fixed Brain Tissue.- Photomarking Relocalization Technique for Correlated Two-Photon and Electron Microcopy Imaging of Single Stimulated Synapses.- 3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and ElectronTomography.- Analyzing Endosomal Docking, Fusion, Sorting, and Budding Mechanisms in Isolated Organelles.- Concurrent Imaging of Receptor Trafficking and Calcium Dynamics by Spinning Disk Confocal Microscopy.- Imaging Activity-Dependent Signaling Dynamics at the Neuronal Synapse Using FRET-Based Biosensors.- Analyzing Structural Plasticity of Dendritic Spines in Organotypic Slice Culture.- Using Fluorescent Markers to Estimate Synaptic Connectivity In Situ.- Dual Anterograde and Retrograde Viral Tracing of Reciprocal Connectivity.- Mapping Synaptic Inputs of Developing Neurons Using Calcium Imaging.- Monosynaptic Tracing in Developing Circuits Using Modified Rabies Virus.

SynopsisThis detailed volume collects protocols for experimentation into how neurons connect to produce the extraordinary functionalities of the nervous system. Contributed by experts and pioneers in their respective techniques, the book covers synapses in the brain and in culture, their constituents, their structures, their dynamics, and the assemblies they form, all in the structure of a laboratory guide. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synapse Development: Methods and Protocols serves as an ideal guide to minimizing the barrier to entry for the integration of new approaches with existing expertise, producing syntheses that will foster novel perspectives on the many ways in which synapses form, transform, and transmit., Part I: Forming Synapses in a Dish 1. A Cell Culture System to Investigate the Presynaptic Control of Subsynaptic Membrane Differentiation at the Neuromuscular Junction Nadine Schmidt, Sreya Basu, Stephan Köger, and Hans Rudolf Brenner 2. Co-Culture Synaptogenic Assay: A New Look at Fluorescence Reporters and Technological Devices Karen Perez de Arce and Massimiliano Stagi 3. Synaptogenic Assays Using Neurons Cultured on Micropatterned Substrates Katalin Czöndör and Olivier Thoumine 4. Monitoring Synapses Via Trans-Synaptic GFP Complementation Theodoros Tsetsenis 5. Generation of Spinal Motor Neurons from Human Pluripotent Stem Cells David P. Santos and Evangelos Kiskinis Part II: Analyzing Synaptic Constituents 6. Biochemical Purification of Binding Partners of Synaptic Scaffold Proteins Bekir Altas, Olaf Jahn, and Hiroshi Kawabe 7. In Situ Protein Binding Assay Using Fc-Fusion Proteins Nirmala Padmanabhan and Tabrez J. Siddiqui 8. Reconstitution of Synaptic SNAREs into Large Liposomes with Reduced Curvature Stress Javier M. Hernandez 9. Isolation of Synaptosomes, Synaptic Plasma Membranes, and Synaptic Junctional Complexes Mary L. Michaelis, Lei Jiang, and Elias K. Michaelis 10. Purification of Synaptosome Populations Using Fluorescence-Activated Synaptosome Sorting Elisa Luquet, Christoph Biesemann, Annie Munier, and Etienne Herzog Part III: Observing Synaptic Structure and Ultrastructure 11. Optimized Protocol for Imaging Cleared Neural Tissues Using Light Microscopy Yoh Isogai, Douglas Richardson, Catherine Dulac, and Joseph Bergan 12. Structured Illumination Microscopy for the Investigation of Synaptic Structure and Function Soyon Hong, Daniel K. Wilton, Beth Stevens, and Douglas S. Richardson 13. 3D d STORM Imaging of Fixed Brain Tissue Frank Herrmannsdörfer, Benjamin Flottmann, Siddarth Nanguneri, Varun Venkataramani, Heinz Horstmann, Thomas Kuner, and Mike Heilemann 14. Photomarking Relocalization Technique for Correlated Two-Photon and Electron Microcopy Imaging of Single Stimulated Synapses Miquel Bosch, Jorge Castro, Mriganka Sur, and Yasunori Hayashi 15. 3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and Electron Tomography Cordelia Imig and Benjamin H. Cooper

LC Classification NumberRC321-580