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About this product
Product Identifiers
PublisherTaylor & Francis Group
ISBN-100367400936
ISBN-139780367400934
eBay Product ID (ePID)6038321932
Product Key Features
Number of Pages648 Pages
Publication NameTechniques in Glycobiology
LanguageEnglish
SubjectChemistry / Analytic, Chemistry / Organic, Chemistry / General
Publication Year2019
TypeTextbook
Subject AreaScience
AuthorArland T. Hotchkiss
FormatTrade Paperback
Dimensions
Item Weight30.3 Oz
Item Length9 in
Item Width6 in
Additional Product Features
Intended AudienceScholarly & Professional
Dewey Edition21
Dewey Decimal572/.567
Table Of ContentMass spectrometry and NMR: new frontiers in nuclear magnetic resonance spectroscopy - use of the Nano.NMR probe for the analysis of microgram quantities of complex carbohydrates; analysis of anionic glycoconjugates by delayed extraction matrix-assisted laser desorption time-of-flight mass spectrometry. Glycolipids and the new physical methods: microscale analysis of glycosphingolipids by thin-layer chromatography blotting; high-performance liquid chromatography of gangliosides; a sensitive cell suspension enzyme-linked immunosorbent assay measuring the density of cell surface ganglioside and carbohydrate antigens. Protein 0-glycosylation: effects of 0-glycosylation on protein structure - the role and characterization of heavily 0-glycosylated mucin domains; predicting mucin-type 0-glycosylation sites; experimental approaches to studying 0-glycosylation in vivo. Labelling oligosaccharides with chromophore and fluorophores: highly sensitive pre-column derivation procedures for quantitative determination of monosaccharides, sialic acids, and amino sugar alcohols of glycoproteins by reversed phase high-performance liquid chromatography; glycan labelling with the fluorophores 2-aminobenzamide and anthranilic acid. Strategies for oligosaccharides separations and sequencing: discovery and uses of novel glucosidases: resolution of oligosaccharides in capillary electrophoresis; a comparison of oligosaccharides profiling methods. Plant glycobiology: recent developments in starch structure analysis using high-performance anion-exchange chromatography with pulsed amperometric detection; red seaweed galactants - methodology for the structural determination of corallinan, a different agaroid. (Part contents)>
SynopsisThis excellent reference covers state-of-the-art methodologies for plant and animal gly-coconjugate analysis-detailing mass spectrometry, nuclear magnetic resonance spectroscopy, glycolipids and new physical methods, O-glycosylation characterization, chromophore and fluorophore labeling of oligosaccharides, separations, exoglycosidases and mapping, and plant glycobiology. Written by over 100 experts from around the world, Techniques in Glycobiology shows how lipid modification with oligosaccharide chains confers unique characteristics to plasma membranes...reveals that protein glycosylation at specific Ser or Thr residues alters polypeptide backbone conformation...presents the determination of O-glycosylation sites with computer algorithms...addresses the use of high resolution separations for assessing the fidelity of glycosylation of recombinant glycoprotein therapeutics...introduces the utilization of DNA sequencers for oligosaccharide chain length analysis...details the analysis of oligosaccharides up to 70 sugar residues long with post-column enzyme reactors coupled to HPAEC-Pad...and more. With over 1300 literature references, tables, equations, drawings, and photographs, this in-depth resource is indispensable for biochemists; biotechnologists; pharmacologists; analytical, pharmaceutical, carbohydrate, and food chemists; molecular biologists; microbiologists; plant physiologists; botanists; and graduate-level students in these disciplines. Book jacket., This work covers methodologies for plant and animal glycoconjugate analysis. It details mass spectrometry, nuclear magnetic resonance spectroscopy, glycolipids and new physical methods, o-glycosylation characterization, chromophore and fluorophore labelling of oligosaccharides, separations, exoglycosidases and mapping, and plant glycobiology.
