This booklet describes the background to the Black and Yellow Sigatoka diseases (causal agents Mycosphaerella fijiensis and M. musicola respectively) and the traditional method of diagnosis by fungal isolation. A new, laboratory based molecular biological technique is presented, which gives rapid and sensitive identification of the pathogens, directly from infected banana leaves. The method, developed at NRI, is based on the amplification of fungal DNA by the Polymerase Chain Reaction (PCR). Protocols for DNA extraction and PCR are given, together with details of reagents and their availability. Suitable for use in plant pathology laboratories equipped for basic molecular biology by researchers who require accurate identification to species level for these pathogens of significant economic importance.
Product Identifiers
Publisher
Natural Resources Institute
ISBN-13
9780859544757
eBay Product ID (ePID)
88008172
Product Key Features
Subject Area
Natural Science
Author
A. Johanson
Publication Name
Detection of Sigatoka Leaf Spot Pathogens of Banana by the Polymerase Chain Reaction